Fig. 2: Neutralization breadth and potency of bispecific antibody G7-Fc.

a Schematic diagrams of the structure of bispecific antibodies. b Cross-linking sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and SDS-PAGE analysis of G7-Fc, 7G-Fc, 7F3, GW01. Binding specificities (c), binding affinities KD values (d) of the bispecific antibodies G7-Fc and 7G-Fc to the RBD-His proteins of WT, Delta, BA.1, BA.5, BF.7, BQ.1.1, XBB, XBB.1.5, XBB.1.16 as measured by ELISA and BLI experiments. ND represents not determined. e Neutralization breadth and potency of bispecific antibodies G7-Fc and 7G-Fc against the SARS-CoV-2 variants and sarbecoviruses. LY-CoV1404 was used as a control. The number in the box indicates IC50 values. Geometric mean (GM) IC50 was determined. f Neutralization of authentic viruses BQ.1 and XBB.1 by G7-Fc. The curves were fitted using nonlinear regression (log [inhibitor] vs. normalized response, variable slope). The dots on the curve represent the mean values of triplicate experiments, accompanied by bars indicating the standard error of the mean (SEM). The experiments in b, e were independently performed twice with consistent results. Source data are provided in the Source Data file.