Fig. 2: Super-resolution fluorescence imaging of S. acidocaldarius adhesion pili dynamics at high temperature. | Nature Communications

Fig. 2: Super-resolution fluorescence imaging of S. acidocaldarius adhesion pili dynamics at high temperature.

From: Adhesion pilus retraction powers twitching motility in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius

Fig. 2

Surface proteins of ∆upsE∆arlJ S. acidocaldarius cells were labeled non-specifically with AlexaFluor 568 NHS-ester. Glass-adhered cells were imaged in Structured Illumination Microscopy (iSIM) every 500 ms. Shown are selected stills from representative movies. a Aap pili mediate dynamic adhesion and twitching motility in S. acidocaldarius. b Pilus retraction mediate dynamic cell-cell interactions in S. acidocaldarius. c Single Aap pilus retraction events were used to measure pilus retraction speed. Retraction speed is given as the difference between final (Lfinal) and initial (Lini) pilus length over the difference between tfinal and tini. d Mean retraction speed in single-pilus retraction events in 19 cells. Superimposed yellow circles represent average values from each of n = 3 independent experiments. Error bars represent the mean ± SEM of those three biological replicates. e Retraction speed is not correlated with pilus length. Scale bars, 2 µm. Source data are provided as a Source Data file.

Back to article page