Fig. 3: Automated tracking of S. acidocaldarius Aap mutants. | Nature Communications

Fig. 3: Automated tracking of S. acidocaldarius Aap mutants.

From: Adhesion pilus retraction powers twitching motility in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius

Fig. 3

a Map of the Aap pilus operon in S. acidocaldarius. b Representative examples of tracks generated for indicated Aap mutants. Arrowheads denote long aligned tracks generated by detaching cells. Scale bars = 5 µm. c Track displacement measured over five minutes for each indicated strain (ΔaapA: N = 418, ΔaapB: N = 302, ΔaapX: N = 427). d Distribution of individual track displacements in the indicated strains. Tracks with a displacement <2 µm were defined as non-motile. e Track persistence ratio in the indicated strains. Superimposed yellow circles represent sample means from each of n = 3 (ΔaapA, ΔaapX) or n = 2 (ΔaapB) independent experiments. Error bars represent the mean ± SEM of those biological replicates for ΔaapA and ΔaapX. Source data are provided as a Source Data file.

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