Fig. 5: Summary of in-cell, biochemical, and stability properties of designed variants.
From: Simultaneous enhancement of multiple functional properties using evolution-informed protein design
[Blue color scheme]: Summary values from multiple independent resistance assays of the minimum inhibitory concentration (MIC) in E. coli of multiple β-lactams. Numbers for each assay are as follows: [broth microdilution] aggregate of three or five replicates (Methods), [colony formation] mean of three replicates, [MIC Strip] mean of three replicates unless any replicate was above the highest tested dose at which point the mode is displayed if available otherwise the median is depicted. Resistance assay colors are log normalized; darker colors: larger values. [Green and red color scheme]: in-vitro biochemical analysis of each design’s ability to hydrolyze ampicillin (green, mean of three replicates) or nitrocefin (green, model parameters fit to three replicates) as well the change in melting temperature (thermostability, mean of three replicates) from WT TEM-1 (red). N/A: the design could not be purified; N/H: the design had no hydrolysis. Biochemical colors are linear; darker colors: larger values. Source data are provided in the Source Data file.
