Fig. 2: The expression of blaOXA-48 induces collateral sensitivity.
From: β-lactamase expression induces collateral sensitivity in Escherichia coli
a Scheme depicting the design and construction of the pUN4 derivative vectors and the pOXA-48TET. Left branch: the region encoding the blaOXA-48 gene was amplified from the pOXA-48WT plasmid and cloned into the pUN4 backbone, obtaining the pUN-OXA-48WT vector. A parallel procedure was carried out for the pOXA-48DEL plasmid, leading to the pUN-OXA-48DEL vector. Right branch: the region encoding the tetC gene was amplified and cloned into the backbone of the pOXA-48WT plasmid, replacing blaOXA-48 and resulting in the pOXA-48TET plasmid. b MIC determination for E. coli J53 carrying the pOXA-48TET plasmid and the pUN4 derivatives in four antibiotics: AMC: amoxicillin-clavulanic acid, ERT: ertapenem, COL: colistin, and AZI: azithromycin. Horizontal lines indicate the median values and each datapoint represents a biological replicate ( n = 6 for ERT and AMC; n = 9 for AZI and COL except for pOXA-48TET, in which n = 6). Strains carrying non-functional variants of the blaOXA-48 gene are represented in red, strains with fully functional blaOXA-48 gene versions in blue, and β-lactamase-free strains in dark grey. Data points have been slightly jittered to avoid overlapping. Asterisks denote statistically different susceptibility levels compared to plasmid-free J53 (*p < 0.05, **p < 0.01, ***p < 0.001; two-sided Mann-Whitney U test). Source data are provided as a Source Data file.
