Fig. 3: Production of different β-lactamases induces CS to AZI and, to some extent, to COL.
From: β-lactamase expression induces collateral sensitivity in Escherichia coli
a The molecular class and distinctive substrate of each β-lactamase are listed in the table. β-lactamases were cloned under the control of the PBAD promoter in the pBAD18 plasmid. b Heat map representing collateral responses to antibiotics associated with β-lactamase acquisition (median of six biological replicates). The colour code represents the fold change in MIC of each antibiotic for the β-lactamase-carrying derivatives relative to E. coli BW25113 strain carrying pBAD18 without β-lactamase. Asterisks denote statistically significant differences (two-way ANOVA followed by Dunnett’s test, adjusted p < 0.047 in all cases). CTX, cefotaxime; AMC, amoxicillin-clavulanic acid; AZM, aztreonam; IMP, imipenem; MER, meropenem; ERT, ertapenem; TET, tetracycline; TMP, trimethoprim; RIF, rifampicin; CIP, ciprofloxacin; FOS, fosfomycin; CHL, chloramphenicol; COL, colistin; and AZI, azithromycin. Source data are provided in Source Data file.
