Fig. 1: Overview of isolation and downstream analysis of cells based on the amount of EV secretion.
From: Optimizing cell therapy by sorting cells with high extracellular vesicle secretion
A Cells are loaded in nanovials (blue) that are conjugated with anti-CD63 capture antibodies and allowed to secrete EVs. B Secreted EVs from each cell are captured by the surrounding nanovial and labeled with a fluorescent antibody against CD9 on the surface of EVs. C Nanovials and corresponding single cells are sorted based on the level of fluorescence signal associated with the captured EVs (High vs. Low Secretors) using a standard cell sorter. D Downstream transcriptomic analysis is performed on the High or Low-secretor cells to characterize gene expression differences. In vivo studies are performed with cells expanded from High Secretor or Low-Secretor populations to characterize the effect on therapeutic outcome. A, B, and D created with BioRender.com, and released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.
