Fig. 5: Reconstructed high-resolution image of hematoxylin and eosin (H&E) stained breast cancer cells. | Nature Communications

Fig. 5: Reconstructed high-resolution image of hematoxylin and eosin (H&E) stained breast cancer cells.

From: High-resolution, large field-of-view label-free imaging via aberration-corrected, closed-form complex field reconstruction

Fig. 5

APIC reconstructed aberration corrected, high-resolution color image is shown on the left. The zoomed image of the highlighted region in the color image is shown on the right. The image with the label “10×” denotes the image acquired using the same 10× magnification objective, which was used for data acquisition and thus showed poorer resolution due to its limited NA. The color image with label “40× (GT)” denotes the ground truth we acquired using a 40× objective whose NA equals the theoretical synthetic NA of APIC. We note that we manually focused the image under red, green, and blue LED illumination when acquiring the ground truth as the best focal planes for them are different, while no tuning was applied in APIC’s data acquisition. We picked a blue channel as an example in this illustration, and the complex field reconstructions and retrieved aberrations are shown at the bottom of the rounded box. From the zoomed images, APIC shows good correspondence with the ground truth, while FPM is much noisier.

Back to article page