Fig. 5: Nucleotide specificity engineering of CtNTH and AdaV.
From: Discovery and substrate specificity engineering of nucleotide halogenases
a Alignment of residues located between the two phosphate-binding sites in dGMP halogenases and reported dAMP halogenases. b Structural alignment of the productive binding modes for CtNTH/dGMP and AdaV/dAMP, highlighting compared residues 175–177 (green), 197-202 (blue), 271–275 (light yellow), and 300–306 (pink). c HPLC analysis of enzymatic reactions catalyzed by CtNTHH274Y, AdaVWT, and AdaVF303V. The experiments were repeated independently three times with similar results. d Steady-state kinetic analysis of CtNTHWT, CtNTHH274Y, AdaVWT, and AdaVF303V. n.d.: not determined. Source data are provided in Source Data file.
