Fig. 3: HBO treatment enhances the tumor accumulation of EcN. | Nature Communications

Fig. 3: HBO treatment enhances the tumor accumulation of EcN.

From: Hyperbaric oxygen enhances tumor penetration and accumulation of engineered bacteria for synergistic photothermal immunotherapy

Fig. 3

a Scheme showing the HBO-enhanced accumulation of EcN (using EcN-mCherry for fluorescence tracking) within an MCS. b Confocal images showing the EcN-mCherry-treated MCSs in the absence or presence of HBO treatment (0 or 2 h). c Representative fluorescence microscopic images of the tumor tissue slices collected from tumor-bearing mice post intravenous injection of EcN-mCherry without/with HBO treatment. d Representative photographs showing EcN colonization amounts inside the tumors from the 4T1 tumor-bearing mice at 24/48/72 h post intravenous injection of EcN without/with HBO treatment. The numbers (104, 105, 106) in the image indicated the dilution factors of the original bacterial suspensions. e Quantitative bacterial counts in per gram of tumor tissues. Data are presented as mean ± standard deviation (SD) and analyzed by two-tailed Student’s t test (*P < 0.05, **P < 0.01). n = 3 mice. Source data are provided as a Source Data file.

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