Fig. 5: In vitro ICD and immunostimulation triggered by EcN-cypate-mediated PTT. | Nature Communications

Fig. 5: In vitro ICD and immunostimulation triggered by EcN-cypate-mediated PTT.

From: Hyperbaric oxygen enhances tumor penetration and accumulation of engineered bacteria for synergistic photothermal immunotherapy

Fig. 5

a Scheme showing the DAMP secretion and ICD-induced BMDC maturation. a was created with BioRender.com (publishing license: HO26QF3LQP). Quantification of extracellular release of b ATP and c HMGB1 from the 4T1 cells with different treatments. d Quantification of CRT exposure on the 4T1 cells with different treatments. b–d Data are presented as mean ± SD. n = 3 experimental repeats. Representative immunofluorescence images showing the e CRT and f HMGB1 in 4T1 cells after different treatments. The cell nuclei stained by Hoechst 33342 present blue fluorescence. The red fluorescence in e indicates the presence of HMGB1. The green fluorescence in f indicates the presence of CRT. g Representative flow cytometric analysis results of mature DCs (CD11c+CD80+CD86+) and h corresponding quantitative statistics of mature DCs after different treatments. Data are presented as mean ± SD. n = 3 experimental repeats. “Laser–”: The 4T1 cells were incubated with EcN-cypate (cypate: 5 μg/mL) for 4 h without laser irradiation. “Laser+”: The 4T1 cells were incubated with EcN-cypate (cypate: 5 μg/mL) for 4 h and then irradiated by an 808 nm laser (1 W/cm2, 5 min). Statistical significance in b–d and h was calculated via one-way analysis of variance (ANOVA) with a Tukey’s post-hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. “ns” stands for nonsignificant difference. Source data are provided as a Source Data file.

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