Fig. 6: Evaluation of the biodistribution and in vivo PTT effect of EcN-cypate. | Nature Communications

Fig. 6: Evaluation of the biodistribution and in vivo PTT effect of EcN-cypate.

From: Hyperbaric oxygen enhances tumor penetration and accumulation of engineered bacteria for synergistic photothermal immunotherapy

Fig. 6

a In vivo and ex vivo distributions of cypate at different time points (24, 48, and 72 h) post intravenous injection of cypate or EcN-cypate. “EcN-cypate + HBO”: The mice were treated with HBO (1.5 ATA, 2 h) post injection of EcN-cypate. b Semiquantitative distribution results of cypate in the tumors and major organs at 48 h post injection in different groups. Data are presented as mean ± SD. n = 3 mice. The abbreviations in a and b represent different organs/tissues as follows: H: heart, Li: liver, S: spleen, Lu: lung, K: kidneys, and T: tumor. c Representative immunofluorescence images of HIF-1α expression in tumor slices. This experiment was repeated for three times independently with similar results. d Representative thermal images of tumor-bearing mice with different treatments after receiving NIR laser irradiation (808 nm, 1 W/cm2) for different time periods. e Temperature changes of the tumor areas in d. The doses of cypate in free cypate, EcN-cypate, and “EcN-cypate + HBO” groups were 10 mg/kg. Statistical significance in b was calculated via one-way ANOVA with a Tukey’s post-hoc test. **P < 0.01, ***P < 0.001, ****P < 0.0001. Source data are provided as a Source Data file.

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