Fig. 3: Coexistent ARG-MRG subtypes and associated transcriptional activities in agricultural soil microbiomes. | Nature Communications

Fig. 3: Coexistent ARG-MRG subtypes and associated transcriptional activities in agricultural soil microbiomes.

From: Organic fertilization co-selects genetically linked antibiotic and metal(loid) resistance genes in global soil microbiome

Fig. 3

a Coexistence network between ARG and MRG subtypes, with node size indicating the presence frequency and edge width representing the coexistence frequency of ARG/MRG subtypes on contigs. b Representative arrangements of AMCCs on plasmids carrying arsenic resistant (acr3, arsC2, arsH, arsR3), transposon (Tn6183, Tn6302), and sulfonamide resistant (sul2) genes (identity and query breadth coverage by amino acids greater than 90%). Relative abundance (c) and transcriptional activity (d) of AMCCs in paddy soils with relatively high and low arsenic stress. e Recruitment plot of reads from the metatranscriptomic datasets originating from CD_BY_2 (top), ZJ_QJ_1 (middle), and CD_BY_1 (bottom) mapped against the assembled AMCCs. In Fig. 4a, b, e, different colors represented different gene types. Source data were provided as a Source Data file. ARG antibiotic resistance gene, MRG metal(loid) resistance gene, MGE mobile genetic element, AMCC ARG-MRG-carrying contigs.

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