Fig. 5: Characterization of ARG-MRG-carrying metagenome-assembled genomes (MAGs). | Nature Communications

Fig. 5: Characterization of ARG-MRG-carrying metagenome-assembled genomes (MAGs).

From: Organic fertilization co-selects genetically linked antibiotic and metal(loid) resistance genes in global soil microbiome

Fig. 5

a Phylogenetic tree of ARG-MRG-carrying MAGs (completeness ≥ 70% and contamination ≤ 5%). The text represented the taxonomic classification of each MAG at the Genus level or Family level; otherwise, the MAG was not classified to the Genus level. The colors of MAGs represented the different Class levels to which these MAGs were assigned. Pie charts represent the relative abundance of each MAG in the NOF and OF soils. The numbers of identified ARGs, MRGs, MGEs, and VFGs among the MAGs were shown in the bar plots. b Abundance of different host-MAGs (antibiotic and metal co-resistant bacteria, AMRB) at the Class level in the NOF and OF soils was calculated by the average coverage of all contigs in the MAG and normalized by per genome equivalents (CPG). c Relative proportions of different host-MAGs (AMRB) at the Class level in the NOF and OF soils. In b, c different colors represented different Class levels, consistent with (a). Source data were provided as a Source Data file. ARG antibiotic resistance gene, MRG metal(loid) resistance gene, MGE mobile genetic element, VFG virulence factor genes, MLS macrolide-lincosamide-streptogramin, Im immune modulation, Ss stress survival, Eds effector delivery system, N/Mf nutritional/metabolic factor, Aa/Ca Antimicrobial activity/Competitive advantage, MAG metagenome-assembled genome.

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