Table 1 S. pombe strains and genotypes

From: Sororin is an evolutionary conserved antagonist of WAPL

Strain number

Genotype

MG1

cen2(D107):kan-ura4+-lacO his7+::lacI-GFP

MG2

cen2(D107):kan-ura4+-lacO his7+::lacI-GFP sor1::kanMX

MG3

cen2(D107):kan-ura4+-lacO his7+::lacI-GFP wpl1::natMX

MG4

cen2(D107):kan-ura4+-lacO his7+::lacI-GFP sor1::kanMX wpl1::natMX

MG5

cen2(D107):kan-ura4+-lacO his7+::lacI-GFP eso1-G799D

MG6

cen2(D107):kan-ura4+-lacO his7+::lacI-GFP sor1::kanMX eso1-G799D

MG7

cen2(D107):kan-ura4+-lacO his7+::lacI-GFP mis4-242

MG8

cen2(D107):kan-ura4+-lacO his7+::lacI-GFP sor1::kanMX mis4-242

MG9

sor1-Pk9::kanMX pds5-myc::kanMX

MG10

pds5-myc::kanMX

MG11

sor1-Pk9::kanMX

MG12

sor1-Pk9::kanMX psm3-GFP::natMX

MG13

sor1-D303A-Pk9::kanMX psm3-GFP::natMX

MG14

sor1-GFP::kanMX

MG15

sor1-Flag::kanMX

JG17331

ade6-M216 lys1-37

JG16900

eso1-G799D

JG16897

sor1::kanMX eso1-G799D

JG16904

eso1-G799D sor1::sor1-wt::hphMX

JG16879

eso1-G799D sor1::sor1-F299A::hphMX

JG16881

eso1-G799D sor1::sor1-V302A::hphMX

JG16883

eso1-G799D sor1::sor1-D303A::hphMX

JG16885

eso1-G799D sor1::sor1-Y305A::hphMX

MG13

sor1::TAP::kanMX

MG14

sor1-F299A::TAP::kanMX

MG15

sor1-V302A::TAP::kanMX

MG16

sor1-D303A::TAP::kanMX

MG17

sor1-Y305A::TAP::kanMX

  1. (other auxotrophic markers not scored).