Fig. 2: SPR curves for binding of antibiotics to whole bacterial cells or OMVs. | Nature Communications

Fig. 2: SPR curves for binding of antibiotics to whole bacterial cells or OMVs.

From: Potent activity of polymyxin B is associated with long-lived super-stoichiometric accumulation mediated by weak-affinity binding to lipid A

Fig. 2

Each serial injection profile was recorded as outlined in Fig. 1 with eight serial doubling concentrations with a maximum concentration of 625 nM. Representative SPR sensorgram of polymyxin B binding to (A) wt-OMVs (253 RU loaded) or (B) resistant-OMVs isolated from E. coli PmrAG53E cells (250 RU loaded). Representative SPR sensorgrams of whole cell binding by polymyxin B with (C) wild-type E. coli cells (169 RU loaded) or (D) polymyxin B-resistant E. coli cells expressing mcr-1 (102 RU loaded). Representative SPR sensorgrams of PMBN binding with (E) wt-OMVs (512 RU loaded) and (F) resistant-OMVs isolated from E. coli PmrAG53E cells (681 RU loaded). G Representative SPR sensorgrams of polymyxin B (solid line, 245 RU loaded) or PMBN (dashed line, 206 RU loaded) binding to wt-OMVs in the presence of 32 mM Mg2+. H Representative SPR sensorgrams of brevicidine binding to wt-OMVs (black line, 462 RU loaded) or resistant-OMVs isolated from E. coli PmrAG53E cells (blue line, 422 RU loaded). All traces are representatives of n ≥ 3 independent SPR runs.

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