Fig. 1: MYG1 is an oncogenic gene associated with CRC progression and poor clinical outcomes.

a Enriched KEGG pathways of GSEA in TCGA COADREAD cohort (n = 433). Top 10 metabolic pathways were labeled. Red: metabolic pathways. FDR: False Discovery Rate. b MYG1 expression in normal mucosa and paired tumor samples were tested by western blot (n = 8) followed by quantification. Quantification is for the presented representative blot from n = 3 independent experiments. c Representative image and quantification of MYG1 IHC analysis of CRC tumor and adjacent normal mucosal in NF-CRC1 cohort (n = 149 pairs). Scale bar, 200 μm (main macrophages) and 50 μm (insets). d Kaplan–Meier analysis of overall survival and progression free survival of patients in NF-CRC1 cohort (n = 149 pairs) based on MYG1 expression evaluated in c. e The number of MYG1 CNV events in TCGA COADREAD cohort (n = 611). f The level of MYG1 protein was detected in normal colon mucosa cell line FHC and CRC cell lines by western blot (bottom) and followed by quantification (top). KRAS and APC status of cell lines were labeled below. W, wild type; M, mutation. The samples derive from the same experiment but different gels for MYG1, and another for β-Tubulin were processed in parallel. n = 3 independent experiments. g Transcription levels (log₂ (x + 1) transformed RSEM normalized count) of MYG1 in TCGA COADREAD cohort with KRAS wild type (WT, n = 194) and mutation (MUT, n = 163). h Representative images and quantification of MYG1 IHC analysis in NF-KRAS cohort with KRAS wild type (WT, n = 41) and mutation (MUT, n = 27). Scale bar, 100 μm. Two-sided permutation test for GSEA and two-sided hypergeometric test for KEGG pathway enrichment analysis (a). Paired two-sided Student’s t-test (b). Two-sided Pearson Chi-square test (c). Log-rank test (d). One-way ANOVA, Dunnett’s multiple comparisons test, comparison with FHC (f). Unpaired two-sided Student’s t-test (g). Two-sided Mann–Whitney test (h). p and r values were provided in the figure. Error bars, mean ± SD. See also Supplementary Fig. 1. Source data are provided as a Source Data file.