Fig. 7: Mitochondrial MYG1 inhibits OXPHOS and apoptosis in CRC cells.

a Co-localization of MYG1 and Cyt c in 293 T and HCT116 cells was detected by IF. Mitotracker was used as an indicator of mitochondria. Scale bar, 10 μm (293 T) and 5 μm (HCT116). Representative of 13 images for 293 T and 9 images for HCT116 from n = 3 independent experiments. PCC of at least two ROIs from each image were analyzed and averaged. Dashed box displayed the selected ROI in the images (left). Graphs represent PCC of pairwise co-localization analysis of n = 3 independent experiments (right). b, c SW480 cells stably overexpressing MYG1 were used for immunoprecipitation in total cell lysates (b), cytoplasm (Cyto) and mitochondrial (Mito) (c, the lysis samples derive from the same experiment but different gels for Cyt c and GAPDH, and another for MYG1 and COX4 were processed in parallel). d Representative images of tumor tissues from n = 3 CRC patients and graphs represent PCC of MYG1 and Cyt c co-localization analysis from n = 3 patients (right). Scale bar, 50 μm. e The expression of Cyt c was detected by western blot. f OCR were detected in control and MYG1^M expressed SW480 and HCT116 cells. g Distribution of Cyt c was detected in cytoplasm (Cyto) and mitochondrial (Mito) lysate by western blot. The samples derive from the same experiment but different gels for Cyt c, and another for COX4 and GAPDH were processed in parallel. h Cleaved Caspase 3 and Cleaved Caspase 9 expression were detected by western blot after treatment of 5-Fu (50 μM for 72 h). The samples derive from the same experiment but different gels for Cleaved Caspase 9 and GAPDH, and another for Cleaved Caspase 3 were processed in parallel. i Apoptosis was analyzed by flow cytometry after treatment of 5-Fu (50 μM for 72 h). n = 3 independent experiments (b–c and e–i). Unpaired two-sided Student’s t-test (f). One-way ANOVA, Dunnett’s multiple comparisons test (i). p value was provided in the figure. Error bars, mean ± SD. Source data are provided as a Source Data file.