Fig. 3: PNPLA3 loss induces hepatic steatosis but ameliorates hypertriglyceridemia in mice challenged with a PUFA-enriched diet and sugar water. | Nature Communications

Fig. 3: PNPLA3 loss induces hepatic steatosis but ameliorates hypertriglyceridemia in mice challenged with a PUFA-enriched diet and sugar water.

From: PNPLA3 is a triglyceride lipase that mobilizes polyunsaturated fatty acids to facilitate hepatic secretion of large-sized very low-density lipoprotein

Fig. 3

a Experimental scheme (Biorender) for creation and treatment of PNPLA3-LKO mice. 8–10-week-old PNPLA3fl/fl mice injected with either AAV-TBG-Null (n = 8) or AAV-TBG-Cre (n = 10) were fed COWD and given access to fructose/glucose in drinking water for 6 weeks. b Liver PNPLA3 mRNA expression as analyzed by qPCR. c Gross appearance of liver (scale bar = 1 cm). d Hematoxylin and eosin (H&E) staining of liver sections. e Total liver TG content. f Total plasma TG content. g Liver mRNA expression of lipogenic genes by qPCR. h Liver mRNA expression of ER stress response genes by qPCR. i Immunoblotting analysis of indicated lipolysis, lipogenesis, and ER-stress associated proteins in liver. j Experimental scheme (Biorender) for PNPLA3 ASO experiment. 10-week-old WT mice fed COWD and given fructose drinking water were treated with control or PNPLA3 ASO biweekly for 3 weeks (n = 9/group). k Liver PNPLA3 mRNA expression in ASO mice by qPCR. l Total liver TG content. m Total plasma TG content in ASO mice. Data presented in (e–h) and (k–m) are independent replicates derived from individual mice. Data are represented as mean ± SD. *p < 0.05, **p < 0.01, two-sided t test. Specific p values: e p = 0.00410. f p = 0.0443. k p = 7.73E-06. l p = 0.0389. m p = 0.0272.

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