Fig. 1: The cryo-EM structures of σA-RPo and σB-RPo.
From: Structural basis of promoter recognition by Staphylococcus aureus RNA polymerase
A Domain organization of S. aureus σA and σB. B The consensus sequences of S. aureus σB-dependent promoters. The sequence logo was created on the WebLogo website (http://weblogo.berkeley.edu) using an alignment of all the σB-dependent promoters listed in Supplementary Table 1. The height of the letters is proportional to their frequency. C Sequences of S. aureus rrnB P1 and yabJ promoters, which are recognized by σA and σB, respectively. Green, the −35 element; blue, the −10 element. D, E The electron potential map without B-factor sharpening (D) and the model (E) of σA-RPo. Gray, RNAP core except ε; cyan, ε; yellow, σA; salmon, nontemplate strand DNA; red, template strand DNA; green, the −35 element; blue, the −10 element. F, G The electron potential map without B-factor sharpening (F) and the model (G) of σB-RPo. Gray, RNAP core except ε and δ; cyan, ε; pink, δ; yellow, σB; salmon, nontemplate strand DNA; red, template strand DNA; green, the −35 element; blue, the −10 element.
