Fig. 2: MenT3 and MenT4 are cumulatively essential for M. tuberculosis to survive in oxidative stress and establish infection in guinea pigs.
The growth of wild type and ΔmenT4ΔT3 was compared after exposure to either oxidative (a) or nitrosative (b) or nutritional (c) or acidic (d) stress. The data shown in these panels are mean ± SD of log10 CFU obtained from two (a, c, d) or three (b) independent experiments, each performed with duplicate cultures. e THP-1 macrophages were infected with various strains, and the number of intracellular bacteria was determined at different time points. The data shown in this panel are mean ± SD of log10 CFU obtained from triplicate wells and representative of two independent experiments performed in duplicates or triplicates. The data shown in these panels in mean ± SD of log10 CFU in lungs (f) and spleens (g) of guinea pigs (Duncan Hartley strain) infected with either wild type or ΔmenT4ΔT3 strain at 4- and 8-weeks post-infection. The data shown in these panels in mean ± SD of log10 CFU obtained from 6 animals (except in (f) week 8, wild type n = 4 and in (g) week 4, ΔmenT4ΔT3 n = 5 and week 8, wild type n = 5). The data shown for the 4-week time point are representative of two independent experiments. The data shown for the 8-week time point are obtained from a single experiment. h The data shown in this panel are mean ± SD of total granuloma score in H&E-stained sections of guinea pigs infected with wild type or ΔmenT4ΔT3 strain of M. tuberculosis at 4- and 8-weeks post-infection. The data shown are obtained from 6 animals from a single experiment (except in week 8, wild type n = 4). i This panel shows representative images of H&E-stained sections of lung tissues of guinea pigs infected with either wild type or ΔmenT4ΔT3 strain at 4- or 8-week post-infection. Scale bar, 100 μm. p values depicted on the graphs were assessed using a two-tailed paired t-test. Source Data are provided as a Source Data file.
