Fig. 3: Global transcriptional changes in lung tissues of mice infected with either wild type or ΔmenT4ΔT3 strain at 4 weeks post-infection.
a, b Deletion of menT3 and menT4 attenuates M. tuberculosis growth in lungs and spleens of mice. The data shown in these panels are mean ± SD of log10 CFU in lungs (a) or spleens (b) of infected Balb/c mice at 4- and 8-weeks post-infection obtained from 5 animals. The data shown in these panels for 4-week time point are representative of two independent experiments. The data shown for the 8-week time point are obtained from a single experiment. p values depicted on the graphs were assessed using a two-tailed paired t-test. c–g Host transcriptional profiles of lung tissues from uninfected or mice infected with either wild type or ΔmenT4ΔT3 strain. c Venn diagram depicting correlation of expression profiles obtained from lung tissues of either uninfected or mice infected with parental or ΔmenT4ΔT3 strain at 4 weeks post-infection. d Volcano plot comparing transcription profiles obtained from lung tissues of mice infected with either wild type or ΔmenT4ΔT3 strain at 4 weeks post-infection. The transcripts with increased or decreased expression have been shown as blue or red dots, respectively. The black dots represent the transcripts that remain unchanged and are not statistically different between these two groups. e–g Heat maps showing transcripts with differential expression in mice infected with either wild type or ΔmenT4ΔT3 strain at 4 weeks post-infection. The transcripts with differential expression in mice infected with these strains are involved in either calcium signaling (e) or apoptosis/autophagy (f), or immune response (g). The color intensity in heatmaps represents the log2 value of normalized expression counts. The data shown in (c–g) are obtained from three independent biological replicates. Source Data are provided as a Source Data file.
