Fig. 1: A Harbinger transposon insertion in EIN2 gene is responsible for androecy. | Nature Communications

Fig. 1: A Harbinger transposon insertion in EIN2 gene is responsible for androecy.

From: Harbinger transposon insertion in ethylene signaling gene leads to emergence of new sexual forms in cucurbits

Fig. 1

a Sexual phenotype of andromonoecious melon (CAM_WT), and androecious mutant (CAM106). Scale bar = 1 cm. b Lateral branches showing a hermaphrodite flower in CAM_WT, and a cluster of male flowers in CAM106. c Schematic of sexual morphs. d Number of hermaphroditic or male flowers per node on the main stem (MS), or at the 1st node of lateral branches (LB). Data are mean ± SE. (n  =  8 biological replicates). e Cloning of the a3 locus. ∆ SNP index between andromonoecious and androecious bulks, calculated with a 1-Mb sliding window. Arrows indicate candidate genes on chromosome 8. f Structure of the CmEIN2 gene and the position of AndroPIF insertion. The ein2_SP1 and ein2_SP2 splicing mutations are shown by arrowhead. g Quantitative real-time PCR (qPCR) of CmEIN2 in leaf, hermaphrodite (H) and male (M) flowers of CAM_WT, and CAM106. P-values from an unpaired two-tailed Student’s t-test are indicated. Data are mean ± SE. (n  =  3 biological replicates). Source data are provided as a Source Data file.

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