Fig. 1: Active immunization with MOG_[35-55] induces severe EAE in IgH^[MOG] mice.

a Left, Disease curves and linear regression of males (WT, n = 8, IgH^[MOG], n = 8) and females (WT, n = 10, IgH^[MOG], n = 10) immunized with MOG_[35-55] and monitored for development of EAE. Linear regression was used for statistical analysis. Representative of > 20 experiments conducted in each sex. Right, area under curve (AUC) analysis of disease curves in left panels. Two-way ANOVA was used to assess the contribution of genotype. The contribution of sex was p < 0.9912 and there was no interaction effect between the variables. b Spinal cords from female WT and IgH^[MOG] mice at endpoints were sectioned and stained with hematoxylin & eosin (H&E; immune infiltration) and Luxol fast blue (LFB; myelin). 10X magnification. Representative of n = 4 mice of each genotype from a single immunization. Quantification in Supplementary Fig. 1b. c Draining LN cells were isolated at disease onset from female WT (n = 3) and IgH^[MOG] (n = 3) mice from a single immunization and IFNγ and IL-17 expression were determined by flow cytometry. Gated on live CD4⁺CD44⁺ events. Two-tailed t-test was used. d Female WT or IgH^[MOG] B cells were co-cultured with CellTrace Violet-labeled MOG_[35-55]-specific 1C6 T cells that were pulsed, or not, with the indicated concentrations of MOG_[35-55]. Gate frequencies indicate the percentage of cells undergoing at least one cell division. Representative of cultures derived from individual mice (n = 3 mice of each genotype). Data is from one experiment. e Sera were collected from male WT (n = 5) and IgH^[MOG] (n = 5) mice at endpoints and the concentration of MOG-specific IgG was assessed by ELISA. Representative of one of two immunizations. A two-tailed t-test was used. In all violin plots, horizontal lines represent medians. Each datapoint represents an individual mouse. Exact p-values can be found in the Source Data file. Linked to Supplementary Fig. 1.