Fig. 2: Increased lymphocytic aggregation in the meninges of immunized IgH^[MOG] mice.

a Brain and cerebellar meningeal sections from female MOG_[35-55]-immunized WT (n = 8) or IgH^[MOG] (n = 9) mice were assessed by immunofluorescence (IF) for expression of B220 (red), CD4 (blue), and CD11c (green) at endpoints, and lymphocyte aggregates were enumerated. Data are from two immunizations. Two-tailed t-test was used. In violin plot, horizontal line represents median. Each datapoint represents an individual mouse. b Assessment of the ECM near meninges in the cerebellum was determined by IF. Expansion on the meningeal ECM is shown by fibronectin (green) and PDGFRα/β (blue) which overlap with B220 (red) B cell clusters. Dotted lines outline the meningeal membrane and (*) denotes vascular endothelium. Representative of n = 4 female mice of each genotype. Data are from two immunizations. c Representative IF images of IgH^[MOG] meningeal lymphocytic clusters, stained for CD138 (yellow), AID (green), B220 (red), DAPI (blue). i., CD138⁺B220⁺, ii., CD138^-AID⁺, iii., CD138⁺B220^-, iv., CD138⁺AID⁺. The source meningeal cluster is presented in Supplementary Fig. 2c. Representative of 4 female mice. Data are from two immunizations. Exact p-values can be found in the Source Data file. Linked to Supplementary Fig. 2.