Fig. 4: CNS IgH[MOG] B cells upregulate IL-23.
From: Myelin-reactive B cells exacerbate CD4+ T cell-driven CNS autoimmunity in an IL-23-dependent manner
a Male WT (n = 6) and IgH[MOG] (n = 6) mice were immunized with MOG[35-55] and B cells were isolated from CNS tissues at endpoints. Expression of IL-23 heterodimer was assessed by intracellular flow cytometry. Data are presented from one of four immunizations. Two-tailed t-test was used. b IF detection of IL-23 (green), B220 (red), CD4 (aqua), DAPI (blue) in the meninges of female MOG[35-55]-immunized WT or IgH[MOG] mice. Representative of n = 3 mice of each genotype from a single immunization. c Female IgH[MOG] mice were immunized with MOG[35-55] and were treated on day (-1) and day 6 with 1 mg anti-p19 (n = 5) or rIgG1 isotype (n = 5). Disease curves representative of one of seven experiments. Two-tailed t-test was used. d Cerebellar meningeal sections from MOG[35-55]-immunized female IgH[MOG] mice, treated with isotype (n = 6) or anti-p19 (n = 6), were assessed for lymphocyte aggregation. Data are from two immunizations. Two-tailed t-test was used. e CNS-infiltrating mature, transitional and class-switched B cells were assessed by flow cytometry from anti-p19 (n = 4) or rIgG1 isotype (n = 4)-treated immunized female IgH[MOG] mice. Gated on live B220+ events. Data are from a single immunization. Two-tailed t-test was used. f CD4+ T cells were isolated from p19 (n = 5) or rIgG1 isotype (n = 5)-treated immunized female IgH[MOG] mice and the indicated cytokines were assessed by flow cytometry. Data are representative of one of two immunizations. Two-tailed t-test was used. g IL23A was assessed from CSF immune cells of MS-affected individuals (see individual information on pwMS in Supplementary Table 1) by qPCR and was correlated to the frequency of CD19+ B cells within the CD45+ CSF leukocyte population of each patient. Linear regression analysis was applied to calculate Spearman r and p-value. In all violin plots, horizontal lines represent medians. Each datapoint represents a biological replicate. Exact p-values can be found in the Source Data file. Linked to Supplementary Fig. 4.
