Fig. 5: Tph cells infiltrate the IgH^[MOG] CNS.

a Male WT (n = 5) and IgH^[MOG] (n = 5) mice were immunized with MOG_[35-55] and T cells were isolated from CNS at endpoints. Expression of PD-1 and CXCR5 was assessed by flow cytometry. Data are representative of seven experiments. Two-tailed t-test was used. b Male WT or IgH^[MOG] mice were immunized and sacrificed at onset (n = 4 each), peak (n = 3 each), recovery (n = 3 each). CNS-infiltrating CD4⁺ T cells were isolated from these groups, as well as from mice that did not develop disease (n = 4 each) and the frequency of PD-1⁺CXCR5^- cells was assessed. Data are from one of two immunizations. Tukey’s post-hoc test after one-way ANOVA was used. c Expression of MHC class II (I–Ag⁷), IL-21, ICOS and CXCL13 were assessed from CNS-infiltrating CD4⁺ T cells taken from immunized male WT (n = 5) and IgH^[MOG] (n = 5) mice, and expression between PD-1^- and PD-1⁺ CD4⁺ subpopulations was compared. Data are representative of two immunizations. Representative plots are presented in Supplementary Fig. 5c. Sidak’s multiple comparisons test after repeated measures two-way ANOVA was used. d Expression of IFNγ and IL-17 was assessed from PD-1^- and PD-1⁺ CD4⁺ T cells from immunized male WT or IgH^[MOG] mice (n = 3 each). Data are representative of two immunizations. Sidak’s multiple comparisons test after repeated measures two-way ANOVA was used. e Male WT (n = 5) and IgH^[MOG] (n = 5) mice were immunized with MOG_[35-55] and T cells were isolated from the dura mater and subdural meninges (SDM) at endpoints. Expression of PD-1 and CXCR5 was assessed by flow cytometry. Data are representative of two experiments for dura mater and one immunization for SDM. Two-tailed t-test was used. f T cells were isolated from spleen and CNS at endpoints from anti-p19 (n = 5) or isotype-treated (n = 4) immunized male IgH^[MOG] mice. Expression of PD-1 and CXCR5 was assessed by flow cytometry. Data are representative of three experiments. Two-tailed t-test was used. In all violin plots, horizontal lines represent medians. Each datapoint represents an individual mouse. Datapoints connected by a line represent paired observations from the same mouse. Exact p-values can be found in the Source Data file. Linked to Supplementary Figs. 5, 6.