Fig. 6: CNS-infiltrating IgH^[MOG] B cells and Tph cells show increased production of reactive oxygen species.

a GO term analysis of the transcriptome of CNS-infiltrating B cells and Tph cells from female IgH^[MOG] (n = 3) vs WT (n = 3) from a single immunization. b Normalized enrichment scores (NES) for “pathways of neurodegeneration” (mmu05022) and “oxidative phosphorylation” (mmu00190) from B cell and Tph cell transcriptomes from the same samples as in (a). The weighted Kolmogorov–Smirnov statistical test with the Benjamini-Hochberg method was used to adjust for the false discovery rate. Uptake of ROS indicator CM-H2DCFDA from B cells (c, e) or Tph cells (d, f) from immunized female IgH^[MOG] vs WT mice (c, d; n = 5 each) or from immunized and anti-p19 (n = 5) vs isotype-treated (n = 6) immunized female IgH^[MOG] mice. Data in c, d are representative of two immunizations. Data in e, f are representative of two immunizations. Two-tailed t-test was used. In all violin plots, horizontal lines represent medians. Each datapoint represents an individual mouse. Exact p-values can be found in the Source Data file. Linked to Supplementary Fig. 7.