Fig. 2: A membrane envelopes the red body.
From: Implicating the red body of Nannochloropsis in forming the recalcitrant cell wall polymer algaenan
A, D + B, E Resin-embedded, heavy metal stained transmission electron microscopy (TEM) of two separate N. oceanica cells preserved by high-pressure freezing at subjective dusk. Presumptive assignments: CW = cell wall, C = chloroplast, RB = red body, N = nucleus. (D) and (E) show the same cells as (A) and (B), respectively, but were acquired at higher magnification. Chloroplasts are identified by stacked groups of photosynthetic thylakoid membranes that converge at the poles of the organelle, and a possible membrane connecting the red body with the chloroplast is indicated with a white arrowhead in (D). C, F A selected slice from the 3D cryo-tomogram capturing the red body with the apparent enveloping membrane (whole series available as Supplementary Movie 1, and details of CLEM/cryoET pipeline in Supplementary Figs. 3 and 4). Both membranes and denser carotenoid contents appear black in the cryo EM density. M = mitochondria. (F) is a digitally enlarged portion of (C) to make the enveloping membrane more visible, particularly for comparison with the nearby mitochondrial membranes. For all images, the scale bar = 500 nm.
