Fig. 3: The formation of the red body is integrated into the diurnal cell cycle of Nannochloropsis.
From: Implicating the red body of Nannochloropsis in forming the recalcitrant cell wall polymer algaenan
A A liquid culture of synchronously dividing cells was sampled at the indicated time points (0 hr = lights on), and cell diameter was quantified by Coulter counter. The resulting distributions are plotted as the relative frequency with the same vertical scaling (bin size ~ 0.01 μm, maximum vertical height ~3.6%, for each distribution, n > 10k). B SIM optical z-sections of cells sampled at the same time as in (A). Chlorophyll autofluorescence (excitation 642 nm, emission >655 nm, pseudo-colored red); green autofluorescence (excitation 488 nm, emission 495 to 550 nm, pseudo-colored green). The Z-position for each cell was chosen to maximize the apparent diameter of the red body. Each individual cell image frame measures 4 × 4 μm. C 3D reconstructions derived from SIM z-stacks of cells chosen to represent stages of the cell cycle. Each 3D rendering is bounded by a 4 × 4 × 4 μm volume, demarcated on the edges of the volume by a white grid.
