Fig. 6: NF2 and ME1 are synthetic lethal in Schwann cells.
From: G6PD and ACSL3 are synthetic lethal partners of NF2 in Schwann cells
A Relative oxidized glutathione levels are increased in NF2-KO Schwann cells after treatment with G6PDi-1 and rescued by n-acetylcysteine (NAC). Bars = mean ± SD. Significance by two-way ANOVA and Tukey’s multiple comparisons test. n = 4 biological replicates. B G6PD inhibition and ME1 inhibition display synthetic lethality in Schwann cells. Cell number (by CellTiter-Glo) of NF2-WT cells treated +/- G6PDi-1 + /- ME1 inhibitor, normalized to the non-treated DMSO condition. Bars = mean ± SD. Significance by one-way ANOVA and Dunnett’s multiple comparisons test. n = 3 biological replicates. C, D Re-expression of ME1 rescues the sensitivity of NF2-KO Schwann cells to G6PD inhibition. C Immunoblot of ME1 levels in NF2-WT cells, NF2-KO cells, and a monoclonal line of NF2-KO cells transfected to express ME1. D Relative cell numbers of the indicated cell lines 4 days after treatment with 25 µM G6PDi-1, normalized to untreated cells. Bars = mean ± SD. Significance by one-way ANOVA and Dunnett’s multiple comparisons test. n = 4 biological replicates. Source data are provided as a Source Data file.
