Fig. 2: Molecular characterization of the NSC-derived neuro- and astrogliogenic lineages within the mouse dentate gyrus and the effects of TBI. | Nature Communications

Fig. 2: Molecular characterization of the NSC-derived neuro- and astrogliogenic lineages within the mouse dentate gyrus and the effects of TBI.

From: Traumatic brain injury promotes neurogenesis at the cost of astrogliogenesis in the adult hippocampus of male mice

Fig. 2

UMAP-based visualization of the major higher-order cell types identified by Seurat in the single-cell dataset. Each dot represents a single cell. Cells with similar molecular profiles group together. Cell types were assigned according to the expression of specific marker genes and are labeled in different colors (a). UMAP-based visualization of single-cell data according to experimental origin (Control or TBI, 5–6 animals per condition) (b). Bar plots showing the relative number of cells per cluster originating from Control or TBI samples against their predicted abundance (62.96% of all cells originating from TBI samples: dashed line), NSC, *P = 4.69 × 10−2; Astrocyte linage, *P = 5.99 × 10−35; Oligo, *P = 2.92 × 10−3; Endo, *P = 5.14 × 10−5; Mgl1, *P = 2.13 × 10−2; Mural, *P = 1.82 × 10−8 vs. expected abundance, two-sided binomial test. A summary of cell numbers and statistical analyses performed is given in Supplementary Data 1 (c). UMAP-based visualization of NSC-derived neuronal and astrocytic lineages obtained by extraction of neuronal and astrocytic cells followed by reclustering. Each dot represents an individual cell and discrete cell states are identified in specific colors (d). UMAP-based visualization of reclustered data according to experimental origin (Control or TBI) (e). Bar plots showing the relative number of cells per identified cell cluster in Control or TBI samples, against their expected abundance (57.39% of all cells originating from TBI samples: dashed line), N-stage 1, *P = 4.66 × 10−2; N-stage 2, *P = 4.07 × 10−4; N-stage 4, *P = 2.97 × 10−4; N-stage 5, *P = 2 × 10−5; A-stage 1, *P = 2.23 × 10−7; A-stage 2, *P = 3.39 × 10−15; A-stage 4, *P = 2.12 × 10−6 vs. expected abundance, two-sided binomial test. A summary of cell numbers per population and statistical analyses performed is given in Supplementary Data 2 (f). GO biological pathway matrix for the cell clusters included in the neuronal (g) and astrocytic (h) lineages. Source data are provided as a Supplementary Data file.

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