Fig. 2: C to T mutations in iPS cells. | Nature Communications

Fig. 2: C to T mutations in iPS cells.

From: iPS cell generation-associated point mutations include many C > T substitutions via different cytosine modification mechanisms

Fig. 2

a Variation in the number of point mutations in human iPS cell lines. The X-axis shows the names of the iPSC lines. Red: 14 iPSCs established from cord blood using all-in one episomal vectors (CB-epi iPSCs); green: 3 iPSCs established from HDF using retrovirus vectors (R-HDFa-iPSCs); orange: 5 iPSCs established from HDF with episomal vectors (E-HDFa-iPSCs); and blue: 53 iPSCs established from HDF with Sendai virus vectors (HipSci iPSCs). The total numbers of SNVs are shown on the Y-axis. C > T transitions are denoted in red. b Mutational profiles of human iPSCs. Mutant bases were classified in 24 different ways in accordance with their 3’-neighbor bases. The Y-axis indicates the numbers of mutations normalized by the genomic abundance of their two contiguous bases, shown as number per 109 bases. Error bars show the SD of the mean. c Profile of germline mutations. d Number of C > T mutations on CpG sequences. Numbers of C > T mutations at CpG sequences are shown as the number of mutations per cell line or per individual. HipSci iPSC (n = 53 biological replicates); E-HDFa-iPSC (n = 5 biological replicates); R-HDFa-iPSCs (n = 3 biological replicates); CB-epi iPSCs (n = 14 biological replicates). Error bars show the SD of the mean. Source data are provided as a Source Data file.

Back to article page