Fig. 5: Effects of Tet1 on C to T mutation in iPSCs. | Nature Communications

Fig. 5: Effects of Tet1 on C to T mutation in iPSCs.

From: iPS cell generation-associated point mutations include many C > T substitutions via different cytosine modification mechanisms

Fig. 5

a Schema for iPS generation with Tet1 forced expression (b) Tet1-iPSC colonies. Tet1-iPSCs: R4FT4-13, 14, 15, 16, 17 and 18. iPSCs (control): R4F- 03, 04 and 05. Scale bar, 50 μm. c Confirmation of retroviral vector integration. Integration of exogenous Tet1 and c-Myc were confirmed by PCR. d iPSC generation frequency. The colony formation rate was analyzed using Nanog-GFP MEFs, and GFP positive colonies were counted (n = 3 technical replicates). Error bars show the SD of the mean. Statistical analysis was performed using two-tailed t-test for three wells each. e Increased CpG C > T mutations frequency due to forced expression of Tet1 during iPSC generation. The number of C > T mutations at CpG sequences is shown. OSKC iPSC lines (n = 3 biological replicates); OSKC+Tet1 iPSC lines (n = 6 biological replicates). Error bars show the SD of the mean. The two-tailed Mann–Whitney-U test was used for statistical analysis. f Mutation profiles of iPSCs (g) CpG C > T and non-CpG C > T mutations in retrotransposon regions. OSKC iPSC lines (n = 3 biological replicates); OSKC+Tet1 iPSC lines (n = 6 biological replicates). Error bars show the SD of the mean. The two-tailed Mann–Whitney-U test was used for statistical analysis. n.s. not significant Source data are provided as a Source Data file.

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