Fig. 5: VTA glutamatergic neurons that project to the NAc shell and LHb are from different sub-populations. | Nature Communications

Fig. 5: VTA glutamatergic neurons that project to the NAc shell and LHb are from different sub-populations.

From: Midbrain glutamatergic circuit mechanism of resilience to socially transferred allodynia in male mice

Fig. 5

a Viral injection schematic and a representative immunofluorescent image showing EGFP-positive VTA neurons (adapted from The Mouse Brain in Stereotaxic Coordinates by Paxinos and Franklin). b A representative in situ hybridization image showing VTA Slc17a6 gene (Vglut2) expression (adapted from Allen Brain Institute ISH data). c, d EGFP-positive fibers in the NAc shell and LHb. e–g Schematic for retrograde tracing strategy, representative immunofluorescent images for viral injection sites in the NAc Shell and LHb. h, i Representative immunofluorescent images and quantitative data of VTA→NAc shell and VTA→LHb projecting glutamatergic neurons. Scale bars are shown in related images. The staining was repeated twice with similar results.

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