Fig. 6: VTA glutamatergic circuits distinctly regulate the development and maintenance of STA resilience.
a Experimental timeline. PWTs and sub-population percentages in BY mice with bidirectional manipulation of the VTA→NAc shell glutamatergic circuit during STA (n = 20, 10, 7, 20, 20 and 16 mice). b BY + mCherry versus BY + Gq, P30min = 0.0044, P2h = 0.0110, P6h = 0.0115; BY + mCherry versus BY + Gi, P30min = 0.4665, P2h = 0.2358, P6h = 0.1384. c Saline versus Gq, P = 0.0248; Saline versus Gi, P = 0.0471; Gq versus Gi, P = 1.3 × 10−5. d, e PWTs and sub-population percentage in BY mice with bidirectional manipulation of the VTA→LHb glutamatergic circuit (n = 10, 9, 9, 20, 9 and 10 mice). f Experimental timeline. g, h PWTs with bidirectional manipulation of the VTA→NAc shell glutamatergic circuit in BY-S (g, n = 20, 10, 16 and 15 mice; BY + mCherry versus BY + Gq, P30min = 0.0013) and BY-R (h, n = 20, 7, 4 and 5 mice) mice, respectively. i, j PWTs with bidirectional manipulation of the VTA→LHb shell glutamatergic circuit in BY-S (i, n = 10, 9, 13 and 6 mice; BY + mCherry versus BY + Gq, P30min = 0.0077) and BY-R (j, n = 10, 9, 7 and 3 mice; BY + mCherry versus BY + Gi, P30min = 0.0019) mice, respectively. k Summary diagram (adapted from The Mouse Brain in Stereotaxic Coordinates by Paxinos and Franklin). Data are expressed as the mean ± s.e.m. *P < 0.05, **P < 0.01, ****P < 0.0001, ns no significance. Data analyzed by (b, d, g, h, i, j) two-sided two-way RM ANOVA with Tukey’s multiple comparisons test, or (c, e) two-sided Fisher’s exact test. Statistical details are presented in Supplementary Table 5. Source data are provided as a Source Data file.
