Fig. 5: H3K4me2 is a specific persistent mark induced by infection.

A Quantification of H3K4me3 normalized to the segmented nuclei using DAPI signal. Data points are expressed as fold change of mean fluorescence intensity of infected A549 cells (MOI 20) at time β post 1° and PI to UI. Graphs display mean ±SEM from n = 4 to 5 biological replicates. Statistical significance was determined by one-way ANOVA with Fisher’s LSD test (ns = not significant, *p = 0.0208). B Quantification of H3K4me1 normalized to the segmented nuclei using DAPI signal. Data points are expressed as fold change of mean fluorescence intensity of infected A549 cells (MOI 20) at time γ post 1° and PI to UI. Graphs display mean ±SD from n = 3 biological replicates. Statistical significance was determined by one-way ANOVA comparing means with Tukey’s multiple comparison test (ns = not significant, **p = 0.0041). C Experimental set-up with methyltransferase global inhibitor Sinefungin describes in Methods section (β = 24 h, γ = 48 h). D–F Quantification of H3K4me2 at time γ (D), H3K4me1 at time γ (E), H3K4me3 at time β F normalized to the segmented nuclei using DAPI signal. Data points are expressed as fold change of mean fluorescence intensity of infected cells (MOI 20) at 1° to UI. Graphs display the mean values ±SEM from n = 3 biological replicates. Statistical significance was determined by one-way ANOVA comparing means with Tukey’s multiple comparison test (ns = not significant, *p = 0.0323). G Experimental set-up with Calyculin A, described in Methods section (α = 3 h, γ = 48 h). H Quantification of H3K4me2 at time γ, intensity normalized to the segmented nuclei using DAPI signal at time γ post 1° (MOI 20) and UI. Box and whiskers plot with a line denoting the median value from n = 3 biological replicates. Statistical significance was determined by one-way ANOVA comparing means with Tukey’s multiple comparison test (****p < 0.0001). Source data are provided as a Source Data file for A–H.