Fig. 5: NOESY spectra of non-deuterated 80 kDa MSG.

Two-dimensional planes from the 3D ¹³C-¹³C-¹H NOESY spectrum for a I5-¹³C^δ1 and A14-¹³C^β. b I327-¹³C^δ1 and A321-¹³C^β. c A63-¹³C^β and L88-¹³C^δ1. d M415-¹³C^ε and L375-¹³C^δ1. e Methyl groups of isoleucine, leucine, valine, methionine, alanine, and threonine showing NOE cross-peaks in the 3D ¹³C-¹³C-¹H NOESY spectra are highlighted as cyan spheres on a cartoon representation of Malate Synthase G (MSG) structure (PDB entry:1D8C). f Normalised NOE cross-peak volumes (cross-peak volume/diagonal-peak volume) versus interproton distances. Grey circles represent the normalised NOE cross-peak volumes obtained for individual NOE cross-peaks, whereas blue circles represent the average of normalised NOE cross-peaks volume over interproton-distance intervals of 0.2 Å, i.e. (Sum of normalised NOE cross-peak volumes) / (Number of cross-peaks), within each interproton-distance interval of 0.2 Å. The blue line represents the fitted curve of NOE cross-peaks volume (V ) and interproton distance (r) using the standard equation V = C/r ⁶, where C is a constant.