Fig. 1: Gel-assisted mass spectrometry imaging (GAMSI).
From: Gel-assisted mass spectrometry imaging enables sub-micrometer spatial lipidomics
Schematics showing the principle and generalized workflow of GAMSI. Samples are (1) treated (optionally) with cleavable small-molecule linkers and polymerized to form a superabsorbent hydrogel composite, (2) homogenized and expanded, (3) immobilized onto a sample plate, (4) subject (optionally) to cleavage of the small-molecule linkers to release the analytes via, for example, a photocleavage event (dotted box), and (5) coated with a matrix (if used) and analyzed on a mass spectrometer. The magnified schematics (solid circles) show the reversible tethering/release of biomolecules and molecular probes to/from the hydrogel polymer chains in the sample-hydrogel composite.
