Fig. 2: Lipid GAMSI.

a Fluorescence intensity per normalized unit tissue area of fresh-frozen and GAMSI-processed (PFA/GA-fixed) mouse cerebellum white matter, fluorescently labeled using a phospholipid dye [bar height, mean; black dots, individual data points; error bar, standard error of the mean (SEM); n  =  15 regions of interest (ROIs) from three brain slices from one animal]. The unit tissue area was normalized to the pre-expansion scale. b Averaged mass spectra (m/z = 650-925) of fresh-frozen and GAMSI-processed (PFA/GA-fixed) mouse cerebellum. c Spatial distributions of selected lipids in the fresh-frozen (left) and GAMSI-processed (PFA/GA-fixed, ~3-fold expanded) (right) mouse cerebellum. The instrument pixel size (raster distance) (AB SCIEX 4800) was set at 100 µm. Scale bar: 1 mm (3 mm). Here and after, unless otherwise noted, color scale bars for mass spectrometry images represent the relative intensity of the signals, m/z (mass-to-charge ratio) values are provided for singly charged deprotonated ions [M-H]^-, and scale bars are provided at the pre-expansion scale (with the corresponding post-expansion size indicated in the brackets). The instrument pixel size refers to the physical size of a pixel as determined by the instrument specifications, whereas the effective pixel size is given as the instrument pixel size divided by the sample expansion factor. PC: phosphatidylcholine; PE: phosphatidylethanolamine; PI: phosphatidylinositol. Source data are provided as a Source Data file.