Fig. 3: The affinity and specificity of DGC for targeting CTGF.
From: A probe for NIR-II imaging and multimodal analysis of early Alzheimer’s disease by targeting CTGF
a SPR data indicates the binding kinetics of free DAG (left) and DGC (right) with CTGF. The KD value of DAG is 22.3 μM and that of DGC is 21.9 nM. b Immunoblotting of CTGF was performed in U87MG, SH-SY5Y and CTX TNA2 cells. The CTGF expression levels are analyzed by imageJ software. Data were presented as mean ± SEM from three independent experiments (n = 3). **P < 0.01; Student’s t-test. c Representative confocal laser scanning microscopy (CLSM) fluorescence (red) and visible chromogenic (brown) images of U87MG, SH-SY5Y, and CTX TNA2 cells after DGC labeling. d Representative CLSM images of U87MG cells labeled with DGC (red fluorescence) directly, or blocked with DAG peptide prior to DGC labeling (red fluorescence), or DGC (red fluorescence) co-staining with anti-CTGF antibody (green fluorescence). Data were from three independent experiments and one representative result is shown. Scale bar = 50 μm. Source data are provided as a Source Data file.
