Fig. 4: DGC multimodally analyzes CTGF in early AD brain sections of APP/PS1 mice in vitro.
From: A probe for NIR-II imaging and multimodal analysis of early Alzheimer’s disease by targeting CTGF
a Immunofluorescence staining of FITC-labeled CTGF antibody (green) and DGC (red) on APP/PS1 mouse brain sections and the age-matched wild-type (WT) brain sections from 1- to 9-month-old. The merge pictures show the colocalization of DGC and CTGF in brain sections. Scale bar = 50 μm. b In situ fluorescence imaging (red), Au elemental of LA-ICP-TOF-MS imaging, and 3, 3′-diaminobenzidine tetrahydrochloride (DAB) chromogenic (brown) imaging of CTGF in whole brain sections of WT mice (left), 3-month-old APP/PS1 mice (middle) and 9-month-old APP/PS1 mice (right). Inset pictures are enlarged areas of DAB chromogenic images. Scale bar = 50 μm. c CLSM fluorescence images of cortex region in brain sections of WT, 3-month-old APP/PS1 mice and 9-month-old APP/PS1 mice stained with DGC (red fluorescence) and FITC-labeled CTGF antibody (green fluorescence). Scale bar = 20 μm. Inset images show the colocalization of DGC and CTGF antibody. Scale bar = 10 μm. d CLSM fluorescence images of DGC (red fluorescence) and FITC-labeled-Aβ antibody (green fluorescence) staining show partial colocalization in cortex region in brain sections of WT, 3-month-old APP/PS1 mice and 9-month-old APP/PS1 mice. Scale bar = 50 μm. The inset pictures show the partial colocalization of DGC and Aβ antibody in 9-month-old APP/PS1 mice. Scale bar = 25 μm. Data were from three independent experiments.
