Fig. 5: Detection of other cancer-related gene indels using the CoHIT system.
From: CoHIT: a one-pot ultrasensitive ERA-CRISPR system for detecting multiple same-site indels
a The upper panel shows the genomic location of the KIT gene p.W557_K558del mutation, and the blue blocks represent exons. The middle panel lists the sequences of the variants-targeted crRNA and the DNA of the WT and two deletion variants. PAM is marked in green, and DNA bases mismatched to the crRNA are marked in red. The chart below shows the time–course results of CoHIT detection of two variants, the WT, and NC. The included fluorescence image is the naked-eye result under blue light after 30 min of reaction. b, c Results of CoHIT detection of the BRAF p.V487_T491del variants and EGFR p.E746_A750del variants. Similar information to that in Fig. 5a is shown. Source data are provided as a Source Data file.
