Fig. 6: Detection of clinical samples and MRD using the CoHIT system.

a Template input assay of the CoHIT system using blood genomic DNA of three NPM1-mutated AML patients, including P9 with 12.3% c.863_864insTCTG, P33 with 39.9% insCATG, and P111 with 44.0% insTCTG. b Statistical table of the sensitivity and specificity of the CoHIT method in detecting 108 AML patients compared with FGS and using NGS as a standard reference. c Detection results of representative clinical samples (P1, P9, P33, P37, P48, P65, and P67) using the NGS, FGS, and CoHIT methods. The genotypes and mutation rates detected by NGS are shown at the top. FGS peak diagrams are shown in the middle, with the mutated bases underlined. The naked-eye results and time course of CoHIT detection are shown below. d Flowchart of the MRD experiment. e CoHIT result for Patient-MRD-1. The sampling dates and naked-eye results are shown for each sample. Values and error bars reflect the means and s.d. of three biological replicates. P values compared with healthy WT control (HC) are determined by two-tailed Student’s t-tests. The asterisks indicate the ranges (*P < 0.05, **P < 0.005, ***P < 0.0005, ns no significance.). The respective P values in left-to-right order are 0.005, 0.4734, 0.8067, 0.8209, 0.9333, 0.019, 0.0117, 0.0015. f NGS and FGS results of Patient-MRD-1 samples dated 10/2021, 06/2022, 10/2022, and 01/2023. g CoHIT and NGS results for Patient-MRD-2 and 3. Values and error bars reflect the means and s.d. of three biological replicates. P values compared with HC are determined by two-tailed Student’s t-tests. The asterisks indicate the ranges (*P < 0.05, **P < 0.005, ***P < 0.0005, ns no significance.). The respective P values in left-to-right order are 0.0025, 0.0048, 0.6713, 0.2075, 0.0321, 0.0003, 0.0009, 0.3449, 0.0009. The percentages are the insTCTG mutation rates determined by NGS. Source data are provided as a Source Data file.