Fig. 7: CoHIT-LFA system and microfluidic chip-based CoHIT detection.
From: CoHIT: a one-pot ultrasensitive ERA-CRISPR system for detecting multiple same-site indels
a Detection principle of the LFA strip for the CoHIT system. b Biotin-ssDNA-FAM concentration optimization for the LFA assay. c Flowchart of the CoHIT-LFA assay. d Optimization of the CoHIT reaction time for the CoHIT-LFA assay. W, WT sample. M, mutant sample. e Detection of the NPM1 gene c.863_864 4-bp insertion in ten AML patient samples. The percentages are mutation rates. f Flowchart of the microfluidic chip-based multiplexing CoHIT detection. IC, inner control. g Detection of 1E6 copies of mutated fragments-containing plasmid in 100 ng WT genomic DNA. h Detection of Target 2 at frequencies of 10%, 1%, and 0.1%. i Detection in clinical samples from six AML patients. Source data are provided as a Source Data file.
