Fig. 3: METTL3 decrease restores SG recovery rate in ALS cellular models.

a Representative images of the indicated SK-N-BE cells after 1 h stress followed by 4 h recovery. G3BP1 antibody staining is depicted in green, FUS-Flag in red. The nuclei are stained with DAPI (blue). The merge of the signal is shown. The scale bar is 20 µm. (n = 4 biologically independent replicates) b Box plots showing the percentage of unrecovered indicated SK-N-BE cells after 1 h stress followed by 4 h recovery. The box plots are defined by minima, 25% percentile, median, 75% percentile, and maximum (See Source Data file for values). The red dot indicates the average percentage in each sample. Each black dot represents the percentage in a single field. Seven fields were acquired for each biological replicate. n = 4 biologically independent replicates. The ratio of each sample versus its experimental control was tested by a two-tailed Student’s t-test. P values are indicated. c Box plots showing the percentage of unrecovered SK-N-BE cells either overexpressing METTL3 or downregulated for ALKBH5 after 1 h stress followed by 4 h recovery. The box plots are defined by minima, 25% percentile, median, 75% percentile, and maximum (See Source Data file for values). The red dot indicates the average percentage in each sample. Each black dot represents the percentage in a single field. Seven fields were acquired for each biological replicate. n = 3 biologically independent replicates. The ratio of each sample versus its experimental control was tested by a two-tailed Student’s t-test. P values are indicated. Source data are provided as a Source Data file.