Fig. 1: Tomato ETI response of P. syringae alleles that elicit ETI on A. thaliana.

Growth of PtoDC3000 strains carrying one of 21 type III effectors known to elicit ETI on A. thaliana Col-0^23,24. Growth data was normalized based on the growth of the virulent control PtoDC3000::Empty Vector (EV). PtoDC3000::HopAB1n served as a positive control for ETI elicitation. The green boxes indicate effectors that caused a significant reduction in bacterial growth compared to the EV (ANOVA post-hoc Tukey-test [2-tailed], P < 0.05). The box plots represent pooled data from four (HopBA1a, HopZ1a, HopAR1h and HopAA1q) or three independent experiments, each with six to 12 replicates (n = 30 for HopBA1a, HopZ1a and HopAR1h, n = 24 for HopAA1q, n = 18 for the rest of effectors). Error bars representing SEM. The dots show individual values, while the boxes display the first quartile, median, and third quartile, with whiskers extending to the smallest and largest values. Data represent the normalized results of twelve experiments. The minimum growth observed for the EV strain was 6.72 log CFU/cm², the maximum was 7.32 log CFU/cm², with an average of 7.12 log CFU/cm². For normalization within a single experiment, the growth of a particular replicate was divided by the average growth of the empty vector strain within that specific experiment.