Fig. 4: The linker region of the FH2 domain determines actin isoform specificity of DIAPH1 and 3 in cellula. | Nature Communications

Fig. 4: The linker region of the FH2 domain determines actin isoform specificity of DIAPH1 and 3 in cellula.

From: The DIAPH3 linker specifies a β-actin network that maintains RhoA and Myosin-II at the cytokinetic furrow

Fig. 4

a Micrographs of HeLa cells expressing GFP-DIAPH1, GFP-DIAPH1 containing the DIAPH3 linker (GFP-DIAPH1-3L), GFP-DIAPH3, and GFP-DIAPH3 containing the DIAPH1 linker (GFP-DIAPH3-1L). The presented micrographs are representative of three independent experiments. b Micrographs of HeLa cells expressing GFP-DIAPH1-3L in the presence or absence of endogenous DIAPH1, stained with β- or γ-actin isoform-specific antibodies. The presented micrographs are representative of three independent experiments. c Micrographs of HeLa cells expressing GFP-DIAPH3-1L in the presence or absence of endogenous DIAPH3, stained with β- or γ-actin isoform-specific antibodies. The presented micrographs are representative of three independent experiments. d Top: Cartoon illustrating the subdivision of dividing cells into furrow and polar regions. Bottom: Quantitation of the intensity of the indicated actin isoform staining within the furrow and polar regions of anaphase cells under different conditions. n = 10 cells analyzed across three independent experiments. Data were presented as mean (red bars) ± SD. *p = 1.083 × 10−5 as determined by two-sided Mann–Whitney non-parametric tests.

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