Fig. 5: Correct positioning of β- and γ-actin networks is required for successful cytokinesis. | Nature Communications

Fig. 5: Correct positioning of β- and γ-actin networks is required for successful cytokinesis.

From: The DIAPH3 linker specifies a β-actin network that maintains RhoA and Myosin-II at the cytokinetic furrow

Fig. 5

a Quantitation of multinucleate cells formed upon expressing GFP-DIAPH3 or GFP-DIAPH3-1L with or without endogenous DIAPH3. For panels a, b: data were presented as mean (solid bar) ± SD (error bars); n = 900 cells scored per condition across three independent experiments. “ns” = 0.085, *p = 1.96 × 10−3, **p = 8.97 × 10−4, ***p = 3.07 × 10−4, ****p = 2.22 × 10−4 as determined by two-sided Mann–Whitney non-parametric tests. b Quantitation of multinucleate cells formed upon expressing GFP-DIAPH1 or GFP-DIAPH1-3L with or without endogenous DIAPH1. “ns” = 0.92, *p = 1.86 × 10−3, **p = 1.41 × 10−3, ***p = 5.21 × 10−4 as determined by two-sided Mann–Whitney non-parametric tests. c Cartoons illustrating derivation of ingression and furrow instability indices. d Ingression indices of different cell lines with or without DIAPH3 and DIAPH3-1L expression. For panels d–g: n = 20 cells analyzed across three independent experiments; red bars denote the mean, the lower bound of boxes represent the 25th percentile, the upper bound of boxes represent the 75th percentile, and whiskers denote minima and maxima. For panels d, f: *p = 4.27 × 10−3, **p = 3.21 × 10−3, ***p = 1.34 × 10−7, ****p = 1.02 × 10−10, *****p = 5.80 × 10−11, ******p = 1.45 × 10−11 determined by two-sided Mann–Whitney non-parametric tests. e Furrow stability indices of different cell lines with or without DIAPH3 and DIAPH3-1L expression. For panels e, g: “ns” p = 0.34, *p = 1.02 × 10−10, **p = 1.45 × 10−11 determined by two-sided Mann–Whitney non-parametric tests. f, g Ingression indices of cells treated as indicated. h, i Furrow ingression rates of cells treated as indicated. Data were presented as mean (red bars) ± SD. n = 5 cells analyzed for all conditions across three independent experiments, except “GFP-DIAPH1-3L control siRNA -DOX” and “GFP-DIAPH3-1L control siRNA -DOX” where n = 10 and 6 respectively across 5 and 4 independent experiments. For panel h: *p = 0.04, **p = 0.032, ***p = 7.94 × 10−3 determined by two-sided Mann–Whitney non-parametric tests. For panel i: ”ns” = 0.38, *p = 0.032, **p = 0.030, ***p = 7.94 × 10−3 determined by two-sided Mann–Whitney non-parametric tests.

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