Fig. 3: Generalization of DVICE under new experimental settings. | Nature Communications

Fig. 3: Generalization of DVICE under new experimental settings.

From: A versatile automated pipeline for quantifying virus infectivity by label-free light microscopy and artificial intelligence

Fig. 3

A Time-resolved analysis of A549 cells infected with AdV-C5-IX-FS2A-GFP with quantification of predicted infection index by DVICE, GFP intensity, and cell confluency. pfu plaque forming units, AUROC area under the receiver operating characteristic curve. Data are presented as means. B Leave-one-out cross-validation of DVICE. Images of wells inoculated with the indicated virus were left out during network training. The performance was evaluated on the left-out images. Data are presented as means and error bars indicate standard deviations. n = 3. C Comparison of DVICE’ performance between two microscopes, including ImageXpress Micro Confocal (IXM-C, Molecular Devices) and Cytation 5 (Agilent). n = 480 (216 infected and 264 uninfected images). Data are presented as means and error bars indicate standard deviations. Source data are provided as a Source Data file.

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